970 980 990 1000 M-DETGKE LVLALYDYQE KSPREVTMKK GDILTLLNST 1010 1020 NKDWWKVEVN DRQGFVPAAY VKKLD
Purity > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.
Supplied as 1 mg/mL solution in sodium citrate 10 mM pH 3.5, NaN3 0.02% , D2O 10%. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 15470 M-1cm-1 calculated). (unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)
Storage -20°C. The protein is stable at 4°C for months. Avoid repeated freeze/thaw cycles. NMR USE: after the experiments return the sample at 4°C.